Fibroblastsaremesenchymalcellsderivedfromtheembryonicmesoderm.Theyhavebeenextensivelyusedforawiderangeofcellularandmolecularstudiesastheyareoneofeasiesttypesofcellstogrowinculture.TheirdurABIlityalsomakesthemamenabletoavarietyofmanipulationsrangingfromstudiesemployinggenetransfectiontomicroinjection.Ingeneral,fibroblastssecreteanon-rigidextracellularmatrixwhichisrichintypeIand/ortypeIIIcollagen[1].Thereisevidenceshowingthatfibroblastsinvariousorgansareintrinsicallydifferent[2].Dermalfibroblastsswitchfromaproliferative,migratoryphasetoacontractile,matrix-remodelingphaseduringwoundhealing.Inaddition,theysecretelargequantitiesofhyaluronaninresponsetoinflammatorystimuli[3].

HDF-f-mtfromSciencellResearchLaboratoriesareisolatedfromfetalhumanskin.HDF-f-mtarecryopreservedatP0anddeliveredfrozen.Eachvialcontains>5x10^5cellsin1mlvolume.HDF-f-mtarecharacterizedbytheirspindlemorphologyandimmunofluorescencewithantibodyspecifictofibronectin.HDF-f-mtarenegativeforHIV-1,HBV,HCV,mycoplasma,bacteria,yeast,andfungi.HDF-f-mtareguaranteedtofurthercultureundertheconditionsprovidedbyScienCellResearchLaboratories;however,HDF-f-mtarenotrecommendedforexpandingasmitomycinCtreatmentpreventsfurthercellproliferation.

RecommendedMedium

ItisrecommendedtouseFibroblastMedium(FM,Cat.#2301)forculturingHDF-f-mtinvitro.

Specifications:

美国Sciencell 研究实验室（ScienCell）是一家生物技术公司，其宗旨是研究和细胞和细胞发育相关的实验和治疗的产品。该ScienCell技术提供了高质量的正常人体和动物的各种细胞，细胞培养基和试剂，中补充，细胞源性核糖核酸，基因和蛋白质的研究团体。在ScienCell科学家还在研究和开发细胞治疗策略，将大大改善通过实现细胞的能力，打击贩运退行性疾病的生活质量。我们的总部设在加利福尼亚州圣迭戈，并于1999年设立。