ThiskitsareqPCRreadyina96-wellplateformat,witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"sCDNA.
Note:allgeneandpseudogenenamesfollowtheirofficialsymbolsbytheHumanGenomeOrganizationGeneNomenclatureCommittee(HGNC).
PseudogenesareDNAsegmentsintroducedintothegenomebygeneduplicationorRNAretrotransposition.Themajorityofpseudogenesbearhighsequencesimilaritytotheircorrespondingfunctionalgenes,buthavelostsomeorallofthefunctionality.Sciencell"sGeneQuery™HumanABCC6-ABCC6P1PseudogeneTranscriptionAnalysisqPCRKit(GQP-ABCC6P1)isdesignedtofacilitatethetranscriptquantificationofpseudogeneABCC6P1anditscorrespondingfunctionalgene,ABCC6.HumanABCC6P1pseudogeneandABCC6genearebothlocatedonchromosome16. AlthoughABCC6geneandABCC6P1pseudogenesharehighsequencesimilarity,GQP-ABCC6P1guaranteesthateachprimersetonlyamplifiesthedesignatedgeneorpseudogenewithoutcross-amplificationforprecisequantification.
GeneQuery™pseudogenetranscriptionanalysisqPCRkitsareqPCRreadyina96-wellplateformat,witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"scDNA.Thecarefullydesignedprimersensurethat:(i)theoptimalannealingtemperatureinqPCRanalysisis65°C(with2mMMg2+,andnoDMSO);(ii)theprimersetrecognizesallknowntranscriptvariantsoftargetgene,unlessotherwiseindicated;and(iii)onlyonegeneorpseudogeneisamplified.EachprimersethasbeenvalidatedbyqPCRwithmeltcurveanalysisandgelelectrophoresis.
CatalogNo. | GK803 |
CountryofManufacture | UnitedStates |
ProductCode | GQP-ABCC6P1 |
Size/Quantity | One96-wellplate,one20ulreactionperwell |
ProductUse | Forresearchuseonly.Itisnotapprovedforhumanoranimaluse,orforapplicationinclinicalorinvitrodiagnosticprocedures. |
Storage | Storeat4°Cuponreceipt.Forlong-termstorage(>1year),storetheplateat-20°Cinamanualdefrostfreezer. |
ShippingInfo | Ambienttemperature. |
美国Sciencell 研究实验室(ScienCell)是一家生物技术公司,其宗旨是研究和细胞和细胞发育相关的实验和治疗的产品。该ScienCell技术提供了高质量的正常人体和动物的各种细胞,细胞培养基和试剂,中补充,细胞源性核糖核酸,基因和蛋白质的研究团体。在ScienCell科学家还在研究和开发细胞治疗策略,将大大改善通过实现细胞的能力,打击贩运退行性疾病的生活质量。我们的总部设在加利福尼亚州圣迭戈,并于1999年设立。