ThiskitsareqPCRreadyina96-wellplateformat, witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"sCDNA. 

Note:allgeneandpseudogenenamesfollowtheirofficialsymbolsbytheHumanGenomeOrganizationGeneNomenclatureCommittee(HGNC).

PseudogenesareDNAsegmentsintroducedintothegenomebygeneduplicationorRNAretrotransposition.Themajorityofpseudogenesbearhighsequencesimilaritytotheircorrespondingfunctionalgenes,buthavelostsomeorallofthefunctionality.Sciencell"sGeneQuery™HumanZNF300-ZNF300P1PseudogeneTranscriptionAnalysisqPCRKit(GQP-ZNF300P1)isdesignedtofacilitatethetranscriptquantificationofpseudogeneZNF300P1anditscorrespondingfunctionalgene,ZNF300.HumanZNF300P1pseudogeneandZNF300genearebothlocatedonchromosome5. AlthoughZNF300geneandZNF300P1pseudogenesharehighsequencesimilarity,GQP-ZNF300P1guaranteesthateachprimersetonlyamplifiesthedesignatedgeneorpseudogenewithoutcross-amplificationforprecisequantification.

GeneQuery™pseudogenetranscriptionanalysisqPCRkitsareqPCRreadyina96-wellplateformat,witheachwellcontainingoneprimersetthatcanspecificallyrecognizeandefficientlyamplifyatargetgene"scDNA.Thecarefullydesignedprimersensurethat:(i)theoptimalannealingtemperatureinqPCRanalysisis65°C(with2mMMg²⁺,andnoDMSO);(ii)theprimersetrecognizesallknowntranscriptvariantsoftargetgene,unlessotherwiseindicated;and(iii)onlyonegeneorpseudogeneisamplified.EachprimersethasbeenvalidatedbyqPCRwithmeltcurveanalysisandgelelectrophoresis. 

Specifications:

美国Sciencell 研究实验室（ScienCell）是一家生物技术公司，其宗旨是研究和细胞和细胞发育相关的实验和治疗的产品。该ScienCell技术提供了高质量的正常人体和动物的各种细胞，细胞培养基和试剂，中补充，细胞源性核糖核酸，基因和蛋白质的研究团体。在ScienCell科学家还在研究和开发细胞治疗策略，将大大改善通过实现细胞的能力，打击贩运退行性疾病的生活质量。我们的总部设在加利福尼亚州圣迭戈，并于1999年设立。