ThismaterialisofferedbyShanghaiPrimeGeneBio-Techforresearch,laboratoryorfurtherevaluationpurposes.NOTFORHUMANUSE.
VEGFwasinitiallypurifiedfrommediaconditionedbynormalbovinepituitaryfolliculo-stellatecellsandbyavarietyoftransformedcelllinesasamitogenspecificforvascularendothelialcells.ItwassubsequentlyfoundtobeidenticaltoanindependentlydiscoveredvascularpermeABIlityfactor(VPF),whichwaspreviouslyidentifiedinmediaconditionedbytumorcelllinesbasedonitsabilitytoincreasethepermeabilityofcapillarybloodvessels.ThreemouseCDNAclones,whicharisethroughalternativesplicingandwhichencodematuremousemonomericVEGFhaving120,164,or188,aminoacids,respectively,havebeenidentified.Tworeceptortyrosinekinases(RTKs),Flt-1andFlk-1(themousehomologueofhumanKDR),bothmembersofthetypeIIIsubclassofRTKscontainingsevenimmunoglobulin-likerepeatsintheirextracellulardomains,havebeenshowntobindVEGFwithhighaffinity.TherolesofthehomodimersofKDR,Flt,andtheheterodimerofKDR/FltinVEGFsignaltransductionremaintobeelucidated.Invivo,VEGFhasbeenfoundtobeapotentangiogenesisinducer.

Specifications:

CatalogNo.	125-07
CountryofManufacture	UnitedStates
ProductCode	rMuVEGF165
Size/Quantity	2μg
ProductUse	Thisproductisforresearchuseonly.Itisnotapprovedforuseinhumans,animals,orinvitrodiagnosticprocedures.
Storage	Thislyophilizedpreparationisstableat2-8°C,butshouldbekeptat-20°Cforlongtermstorage,preferablydesiccated.Uponreconstitution,thepreparationisstableforuptooneweekat
ShippingInfo	Dryice
AASequence	MAPTTEGEQKSHEVIKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTSESNITMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPEKHCEPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR
Source	Escherichiacoli.
MolecularWeight	RecombinantmurineVEGF165isa39.0kDadisulfide-linkedhomodimericproteinconsistingoftwo165aminoacidpolypeptidechains.
Purity	>95%bySDS-PAGEandHPLCanalyses.
BIOLOGicalActivity	Measuredbyitsabilitytostimulate3H-thymidineincorporationinHUVEcells.TheED50forthiseffectistypically2-4ng/mL.
PhysicalAppearance	SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation	Lyophilizedfroma0.2?mfilteredsolutioninPBS,pH7.4.
Endotoxin	Lessthan1EU/μgofrmVEGF165asdeterminedbyLALmethod.
Reconstitution	Werecommendthatthisvialbebrieflycentrifugedpriortoopeningtobringthecontentstothebottom.Reconstituteinsteriledistilledwateroraqueousbuffercontaining0.1%BSAtoaconcentrat

美国Sciencell 研究实验室（ScienCell）是一家生物技术公司，其宗旨是研究和细胞和细胞发育相关的实验和治疗的产品。该ScienCell技术提供了高质量的正常人体和动物的各种细胞，细胞培养基和试剂，中补充，细胞源性核糖核酸，基因和蛋白质的研究团体。在ScienCell科学家还在研究和开发细胞治疗策略，将大大改善通过实现细胞的能力，打击贩运退行性疾病的生活质量。我们的总部设在加利福尼亚州圣迭戈，并于1999年设立。