Macrophagesarecellsdifferentiatedfromcirculatingbonemarrow-derivedmonocytes.Inthebonemarrowandsubsequestlyinthebloodandtissues,monocytesundergoacascadeofmaturationthatculminateinmorphologicallyandfunctionallymaturetissuemacrophages[1].MacrophagesfunctiontoremovalcellulardebrisandinvADIngpathogens.Theyphagocytizeinvadingmicroorganisms,andscavengedead,damagedcellsandcellulardebris.MacrophagescanbeidentifiedbythespecificexpressionofseveralcellsurfaceproteinsincludingCD14,CD11b,F4/80(mice)/EMR1(human),MAC-1/MAC-3,andCD68byflowcytometryorimmunohistochemicalstaining[2].

MMa-bmfromSciencellResearchLaboratoriesareisolatedfromadultmousebonemarrow.MMa-bmareharvestedatP0anddeliveredfrozen.Eachvialcontains>1x10^6cellsin1mlvolume.MMa-bmarecharacterizedbyimmunofluorescencewithantibodyspecifictoCD11bandF4/80.MMa-bmarenegativeformycoplasma,bacteria,yeast,andfungi.MMa-bmareguaranteedtofurthercultureundertheconditionsprovidedbyScienCellResearchLaboratories;however,MMa-bmarenotrecommendedforexpandingorlong-termculturessincethecellsdonotproliferateinregularculture.

RecommendedMedium

ItisrecommendedtouseMacrophageMedium(MaM,Cat.#1921)fortheculturingofMMa-bminvitro.

Specifications:

美国Sciencell 研究实验室（ScienCell）是一家生物技术公司，其宗旨是研究和细胞和细胞发育相关的实验和治疗的产品。该ScienCell技术提供了高质量的正常人体和动物的各种细胞，细胞培养基和试剂，中补充，细胞源性核糖核酸，基因和蛋白质的研究团体。在ScienCell科学家还在研究和开发细胞治疗策略，将大大改善通过实现细胞的能力，打击贩运退行性疾病的生活质量。我们的总部设在加利福尼亚州圣迭戈，并于1999年设立。