Includes1vialoffrozenHiPSC-NSC(Cat.#1650,1mL,>1x10^6cells/vial),50mLNeuralInductionMedium-basal(PSCNIM,Cat.#5931),1mLNeuralInductionMediumSupplement50x(PSCNIS,Cat.#5982)

Thisitemwillbeavailabletoshipapproximately3weeksaftertheorderissubmitted

HumanNeuralStemCells(Cat.#1650)fromScienCellResearchLaboratoriesaredifferentiatedfromahumaninducedpluripotentstemcellline(hiPSC),whichisgeneratedusingmRNAreprogrammingtechnologyfromHumanDermalFibroblasts(HDF).ThemonolayerHDF-hiPSCareefficientlyconvertedtoneuralepitheliumusingHPSCNeuralInductionMedium(PSCNIM,Cat.#5931),aserum-freemediumforrapidandefficientneuralinductionofhumanpluripotentstemcells(hPSCs).Synergisticinhibitionofglycogensynthasekinase3(GSK3)andtransforminggrowthfactorb(TGF-b)differentiatesHDF-hiPSCstohomogenousneuralstemcells(NSC)inthepresenceofhumanleukemiainhibitoryfactor(LIF)within7days.ThederivedNSCarecharacterizedbyimmunofluorescencewithantibodiesspecifictonestinandSOX2.Thecellpopulationishighlypure:>90%ofcellsexpressNestinand>80%ofcellsareSOX2positive.Afterreviving,NSCcanbemaintainedinHPSCNeuralInductionMediumasanadherentcultureorformneuralspheresinEGFandbFGFcontainingneuralstemcellmedium.Thecellsareabletoformrosetteandneuraltube-likestructuresafterbeingpassagedandplatedathighdensityonMatrigelcoatedculturevesselsinneuralstemmediumcontainingEGFandbFGF.HiPSC-NSCcanbefurtherexpandedformonthsasamonolayeronMatrigelTMcoatedculturevesselsorinsuspensionasneuralsphereswhenseededonultra-lowbindingculturevesselsinneuralstemcellmedium.NSCaremultipotentandabletodifferentiateintovariousneuronalandglialsubtypes.Specificpatterningcues,suchasSHH,retinoicacidandFGF8,canbeaddedafterrevivingtodirectthecellstodifferentneurallineages.HiPSC-NSCarecryopreservedatpassage0anddeliveredfrozen.Eachvialcontains>1x10^6cellsin1mlvolume.CellsarenegativeforHIV-1,HBV,HCV,mycoplasma,bacteria,yeastandfungi.

Specifications:

CatalogNo.	1650
CountryofManufacture	UnitedStates
ProductCode	HiPSC-NSC
Size/Quantity	1x10^6cells/vial
ProductUse	HiPSC-NSCareforresearchuseonly.Itisnotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures.
Storage	Uponreceiving,directlyandimmediatelytransferthecellsfromdryicetoliquidnitrogenandkeepthecellsinliquidnitrogenuntiltheyareneededforexperiments.
ShippingInfo	Dryice.
References	[1]ChambersSM,FasanoCA,PapapetrouEP,TomishimaM,SadelainM,StuderL.(2009)"HighlyefficientneuralconversionofhumanESandiPScellsbydualinhibitionofSMADsignaling."NatBiotechnol.27(3):275-280.[2]LiW,SunW,ZhangY,WeiW,AmbasudhanR,XiaP,TalantovaM,LinT,KimJ,WangX,KimW,LiptonSA,ZhangK,DingS.(2011)"Rapidinductionandlong-termself-renewalofprimitiveneuralprecursorsfromhumanembryonicstemcellsbysmallmoleculeinhibitors."PNAS.108(20):8299-8304.

美国Sciencell 研究实验室（ScienCell）是一家生物技术公司，其宗旨是研究和细胞和细胞发育相关的实验和治疗的产品。该ScienCell技术提供了高质量的正常人体和动物的各种细胞，细胞培养基和试剂，中补充，细胞源性核糖核酸，基因和蛋白质的研究团体。在ScienCell科学家还在研究和开发细胞治疗策略，将大大改善通过实现细胞的能力，打击贩运退行性疾病的生活质量。我们的总部设在加利福尼亚州圣迭戈，并于1999年设立。