Macrophagesarecellsdifferentiatedfromcirculatingbonemarrow-derivedmonocytes.Inthebonemarrowandsubsequestlyinthebloodandtissues,monocytesundergoacascadeofmaturationthatculminateinmorphologicallyandfunctionallymaturetissuemacrophages[1].MacrophagesfunctiontoremovalcellulardebrisandinvADIngpathogens.Theyphagocytizeinvadingmicroorganisms,andscavengedead,damagedcellsandcellulardebris.MacrophagescanbeidentifiedbythespecificexpressionofseveralcellsurfaceproteinsincludingCD14,CD11b,F4/80(mice)/EMR1(human),MAC-1/MAC-3,andCD68byflowcytometryorimmunohistochemicalstaining[2].
MMa-bmfromSciencellResearchLaboratoriesareisolatedfromadultmousebonemarrow.MMa-bmareharvestedatP0anddeliveredfrozen.Eachvialcontains>1x10^6cellsin1mlvolume.MMa-bmarecharacterizedbyimmunofluorescencewithantibodyspecifictoCD11bandF4/80. MMa-bmarenegativeformycoplasma,bacteria,yeast,andfungi.MMa-bmareguaranteedtofurthercultureundertheconditionsprovidedbyScienCellResearchLaboratories;however,MMa-bmarenotrecommendedforexpandingorlong-termculturessincethecellsdonotproliferateinregularculture.
RecommendedMedium
ItisrecommendedtouseMacrophageMedium(MaM,Cat.#1921)fortheculturingofMMa-bminvitro.
CatalogNo. | M1920-57 |
CountryofManufacture | UnitedStates |
ProductCode | MMa-bm |
Size/Quantity | 1x10^6cells/vial |
ProductUse | MMa-bmareforresearchuseonly.Theyarenotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures. |
Storage | Transfercellsdirectlyandimmediatelyfromdryicetoliquidnitrogenuponreceivingandkeepthecellsinliquidnitrogenuntilcellcultureisneededforexperiments. |
ShippingInfo | Dryice. |
References | [1].ClineMJandSumnerMA.(1972)BoneMarrowMacrophagePrecursors.I.SomeFunctionalCharacteristicsoftheEarlyCellsoftheMouseMacrophageSeries.Blood.40:62-69.[2].WynnTAandBarronL.(2010)Macrophages:masterregulatorsofinflammationandfibrosis.SeminLiverDis.30:245-57.[3].GordonSandTaylorRP.(2005)Monocyteandmacrophageheterogeneity.NatureReviewsImmunology.5:953-964. |
美国Sciencell 研究实验室(ScienCell)是一家生物技术公司,其宗旨是研究和细胞和细胞发育相关的实验和治疗的产品。该ScienCell技术提供了高质量的正常人体和动物的各种细胞,细胞培养基和试剂,中补充,细胞源性核糖核酸,基因和蛋白质的研究团体。在ScienCell科学家还在研究和开发细胞治疗策略,将大大改善通过实现细胞的能力,打击贩运退行性疾病的生活质量。我们的总部设在加利福尼亚州圣迭戈,并于1999年设立。