Macrophagesarecellsdifferentiatedfromcirculatingbonemarrow-derivedmonocytes.ThemainfunctionofmacrophagesistoremovecellulardebrisanddestroyinvADIngpathogens.MouseHepaticMacrophages(MHMa),whicharealsoknownasKupffercells,residewithinthelumenofliversinusoids.MHMaprotecttheliverbyrespondingtopathogensandmetastaticcells,whiletoleratingharmlessselfandforeignantigens,whichenterviabloodflowthroughtheportalveinandhepaticartery[1].Recentstudieshaveshownthathepaticmacrophagesplayanimportantroleinfibrosis,liverinflammation,fattyliverdisease,andlivertransplantation[2-4].MHMaareanexcellentmodelforstudyingmacrophagefunctionsundernormalphysiologicalandpathologicalconditions.
MHMafromScienCellResearchLaboratoriesareisolatedfrompostnatalday2mouseliver.MHMaarecryopreservedafterpurificationanddeliveredfrozen.Eachvialcontains>1x10^6cellsin1mlvolume.MHMaarecharacterizedbyimmunofluorescencewithantibodytoF4/80.MHMaarenegativeformycoplasma,bacteria,yeast,andfungi.MHMaareguaranteedtofurthercultureintheconditionsprovidedbyScienCellResearchLaboratories;however,RHMaarenotrecommendedforexpandingorlong-termculturessincethecellsdonotproliferateinregularculture.
ItisrecommendedtouseMacrophageMedium(MaM,Cat.#1921)forculturingMHMainvitro.
CatalogNo. | M5340 |
CountryofManufacture | UnitedStates |
ProductCode | MHMa |
Size/Quantity | 1x10^6cells/vial |
ProductUse | MHMaareforresearchuseonly.Theyarenotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures. |
Storage | Uponreceiving,directlyandimmediatelytransferthecellsfromdryicetoliquidnitrogenandkeepthecellsinliquidnitrogenuntiltheyareneededforexperiments. |
ShippingInfo | Dryice. |
References | [1]LiaskouE,WilsonDV,OoYH.(2012)"Innateimmunecellsinliverinflammation."MediatorsInflamm.2012:949157.[2]BieghsV,VerheyenF,vanGorpPJ,HendrikxT,WoutersK,LutjohannD,GijbelsMJ,FebbraioM,BinderCJ,HofkerMH,Shiri-SverdlovR.(2012)"InternalizationofmodifiedlipidsbyCD36andSR-AleadstohepaticinflammationandlysosomalcholesterolstorageinKupffercells."PLoSOne.7:e34378.[3]TianY,JochumW,GeorgievP,MoritzW,GrafR,ClavienPA.(2006)"Kupffercell-dependentTNF-alphasignalingmediatesinjuryinthearterializedsmall-for-sizelivertransplantationinthemouse."ProcNatlAcadSciUSA.103:4598-603.[4]SekiE,deMinicisS,InokuchiS,TauraK,MiyaiK,vanRooijenN,SchwabeRF,BrennerDA.(2009)"CCR2promoteshepaticfibrosisinmice."Hepatology.50:185-97 |
美国Sciencell 研究实验室(ScienCell)是一家生物技术公司,其宗旨是研究和细胞和细胞发育相关的实验和治疗的产品。该ScienCell技术提供了高质量的正常人体和动物的各种细胞,细胞培养基和试剂,中补充,细胞源性核糖核酸,基因和蛋白质的研究团体。在ScienCell科学家还在研究和开发细胞治疗策略,将大大改善通过实现细胞的能力,打击贩运退行性疾病的生活质量。我们的总部设在加利福尼亚州圣迭戈,并于1999年设立。