Macrophagesarecellsdifferentiatedfromcirculatingbonemarrow-derivedmonocytes.ThemainfunctionofmacrophagesistoremovecellulardebrisanddestroyinvADIngpathogens.MouseSplenicMacrophages(MSMa)resideinthespleenandplayacriticalroleinmaintainingbloodhomeostasis[1].Therearethreedistinctpopulationsofsplenicmacrophagesincluding:redpulpmacrophages,marginalzonemacrophages,andmarginalzonemetallophillicmacrophages[1,2].MSMaperformironprocessingfunctionsandaidinthecaptureofmicrobesandvirusesinthecirculatorysystem[1,2].Recentstudieshaveshownthatsplenicmacrophagescontributetostrokepathologyandautoimmunediseases[3,4].MSMaareanexcellentmodelforstudyingmacrophagefunctionsundernormalphysiologicalandpathologicalconditions.
MSMafromSciencellResearchLaboratoriesareisolatedfromadultCD-1mousespleen.MSMaarecryopreservedafterpurificationanddeliveredfrozen.Eachvialcontains>1x10^6cellsin1mlvolume.MSMaarecharacterizedbyimmunofluorescencewithantibodytoF4/80.MSMaarenegativeformycoplasma,bacteria,yeast,andfungi.MSMaareguaranteedtofurthercultureintheconditionsprovidedbyScienCellResearchLaboratories;however,MSMaarenotrecommendedforexpandingorlong-termculturessincethecellsdonotproliferateinregularculture.
CatalogNo. | M5550 |
CountryofManufacture | UnitedStates |
ProductCode | MSMa |
Size/Quantity | Eachvialcontains>1x10^6cellsin1mlvolume |
ProductUse | MSMaareforresearchuseonly.Theyarenotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures. |
Storage | Uponreceiving,directlyandimmediatelytransferthecellsfromdryicetoliquidnitrogenandkeepthecellsinliquidnitrogenuntiltheyareneededforexperiments. |
ShippingInfo | Dryice. |
References | [1]BorgesdaSilvaH,FonsecaR,PereiraR,CassadoAdosA,AlvarezJ,D"Imperio,LimaM.(2015)"Splenicmacrophagesubsetsandtheirfunctionduringblood-borneinfections."FrontImmunol.6:480.[2]GammellaE,BurattiP,CairoG,RecalcatiS.(2014)"Macrophages:centralregulatorsofironbalance."Metallomics.6(8):1336-1345.[3]LiuZ,ChenCLiF,ShenJ,YangY,LeakR,JiX,DuH,HuX.(2015)"Splenicresponsesinischemicstroke:newinsightsintostrokepathology."CNSNeurosciTher.21(4):320-326.[4]McGabaT,KarlssonM.(2016)"Apoptoticcellresponsesinthesplenicmarginalzone:aparadigmforimmunologicreactionstoapoptoticantigenswithimplicationsforautoimmunity."ImmunolRev.269(1):26-43. |
美国Sciencell 研究实验室(ScienCell)是一家生物技术公司,其宗旨是研究和细胞和细胞发育相关的实验和治疗的产品。该ScienCell技术提供了高质量的正常人体和动物的各种细胞,细胞培养基和试剂,中补充,细胞源性核糖核酸,基因和蛋白质的研究团体。在ScienCell科学家还在研究和开发细胞治疗策略,将大大改善通过实现细胞的能力,打击贩运退行性疾病的生活质量。我们的总部设在加利福尼亚州圣迭戈,并于1999年设立。