Macrophagesarecellsdifferentiatedfromcirculatingbonemarrow-derivedmonocytes.ThemainfunctionofmacrophagesistoremovecellulardebrisanddestroyinvADIngpathogens.MouseSplenicMacrophages(MSMa)resideinthespleenandplayancriticalroleinmaintainingbloodhomeostasis[1].Therearethreedistinctpopulationsofsplenicmacrophagesincluding:redpulpmacrophages,marginalzonemacrophages,andmarginalzonemetallophillicmacrophages[1,2].MSMaperformironprocessingfunctionsandaidinthecaptureofmicrobesandvirusesinthecirculatorysystem[1,2].Recentstudieshaveshownthatsplenicmacrophagescontributetostrokepathologyandautoimmunediseases[3,4].MSMaareanexcellentmodelforstudyingmacrophagefunctionsundernormalphysiologicalandpathologicalconditions.

MSMafromSciencellResearchLaboratoriesareisolatedfromadultC57BL/6mousespleen.MSMaarecryopreservedafterpurificationanddeliveredfrozen.Eachvialcontains>1x106cellsin1mlvolume.MSMaarecharacterizedbyimmunofluorescencewithantibodytoF4/80.MSMaarenegativeformycoplasma,bacteria,yeast,andfungi.MSMaareguaranteedtofurthercultureintheconditionsprovidedbyScienCellResearchLaboratories;however,MSMaarenotrecommendedforexpandingorlong-termculturessincethecellsdonotproliferateinregularculture.

RecommendedMedium

ItisrecommendedtouseMacrophageMedium(MaM,Cat.#1921)forculturingMSMainvitro.

Specifications:

美国Sciencell 研究实验室（ScienCell）是一家生物技术公司，其宗旨是研究和细胞和细胞发育相关的实验和治疗的产品。该ScienCell技术提供了高质量的正常人体和动物的各种细胞，细胞培养基和试剂，中补充，细胞源性核糖核酸，基因和蛋白质的研究团体。在ScienCell科学家还在研究和开发细胞治疗策略，将大大改善通过实现细胞的能力，打击贩运退行性疾病的生活质量。我们的总部设在加利福尼亚州圣迭戈，并于1999年设立。