Substrateforcellcultureadhesion.Optimalconditionsforattachmentmustbedeterminedforeachcelllineandapplication.Recommendedconcentrationfornormalhumancellattachmentis100-200µg/cm2.
Gelatinisaheterogeneousmixtureofwater-solubleproteinsfromporcineskin.0.2%GelatinSolution(GSN)isusedtocoatthesurfaceofculturevesselstopromotecellattachment.ItisrecommendedforthecultureofdifferentiatedhESCcells[1,2],aswellascertainprimaryandimmortalizedcelltypes.
Concentration
Sterile0.2%gelatinincellculturegradewater.
CatalogNo. | 0423 |
CountryofManufacture | UnitedStates |
ProductCode | GSN |
Size/Quantity | sterile,100ml |
ProductUse | THESEPRODUCTSAREFORRESEARCHUSEONLY.Notapprovedforhumanorveterinaryuse,forapplicationtohumansoranimals,orforuseinclinicalorinvitroprocedures. |
Storage | Productisstablefor2yearsandremainsunchangedfromthedateofreceiptwhenstoredat2-8°C.Keepsterile. |
ShippingInfo | Gelice. |
References | [1].ReubinoffBE,PeraMF,FongCY,TrounsonA,BongsoA.(2000).Embryonicstemcelllinesfromhumanblastocysts:somaticdifferentiationinvitro.NatureBiotechnology.Apr;18(4):399-404.[2].Ko.J,Kolehmainen,K.,Ahmed,F,Jun,M.B.G,Willerth,S.M.(2012).Towardshighthroughputtissueengineering:developmentofchitosan-calciumphosphatescaffoldsforengineeringbonetissuefromembryonicstemcells.AmJStemCell;1(1):81-89. |
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