MouseEmbryonicFibroblast-ConditionedMedium(MEF-cm)isaserumfreemediumforthefeederindependentcultureofhumanpluripotentstemcells(hPSCs),includingembryonicstemcellsandinducedpluripotentstemcells.MEF-cmisanefficientalternativetoMEFfeedercells,supportingtheself-renewalofhPSCandmaintainingpluripotencyinthepresenceofbasicFGFandhPSCqualifiedmatrix(e.g.,BDMatrigelTMmatrix).

Sciencell’sMEF-cmwaspreparedusinghighqualitymitomycinCtreatedMEF(MEF-mt,Cat.#M7570-mt)derivedfromE13embryosofCF1mice.SerumfreemediumwasconditionedwithmitomycinCtreatedCF1MEFsfor24hours.Theconditionedmediumwascollecteddailyfor7days,pooledandsterilizedbyfilteringthrougha0.22µmfilter.

BeforeusingMEF-cmforhPSCculture,pleaseaddfreshrecombinanthumanbasicFGF(4ng/mL)(rhbFGF,Cat.#104-02)intothemediumtomakethecompletemedium.ThecompleteMEF-cmhasbeentestedforitsABIlitytosupporthPSCself-renewalonBDMatrigelTMmatrixinvitro.Themediumcolorofeachlotmayvarybutdoesnotaffectperformance.

AdditionalMaterialsRequired

TherecombinanthumanbasicFGF(rhbFGF,Cat.#104-02)shouldbepurchasedseparately.

Specifications:

美国Sciencell 研究实验室（ScienCell）是一家生物技术公司，其宗旨是研究和细胞和细胞发育相关的实验和治疗的产品。该ScienCell技术提供了高质量的正常人体和动物的各种细胞，细胞培养基和试剂，中补充，细胞源性核糖核酸，基因和蛋白质的研究团体。在ScienCell科学家还在研究和开发细胞治疗策略，将大大改善通过实现细胞的能力，打击贩运退行性疾病的生活质量。我们的总部设在加利福尼亚州圣迭戈，并于1999年设立。