MouseEmbryonicFibroblasts(MEF)areusedtosupportthegrowthofmouseandhumanpluripotentstemcells[1].MEFnotonlyprovideasubstrateforpluripotentstemcellstogrowon,butalsosecretecriticalgrowthfactorstomaintainstemcellpluripotency.MEFareisolatedfrommouseembryosandusedatearlypassages[2].Toserveasfeedercells,MEFmustbetreatedwithmitomycinCorbyirrADIationtopreventcellproliferation.Thetreatedcellscanalsobeusedtogenerateconditionedmediumforfeeder-freecultureofpluripotentstemcells.

MEFfromSciencellResearchLaboratoriesareisolatedfromembryonicday13CD1mouseembryos.Thesecellsarecryopreservedatprimarycultureanddeliveredfrozen.Eachvialcontains1x10^6cellsin1mlvolume.MEFarecharacterizedbyimmunofluorescencewithantibodyspecifictofibronectin.MEFarenegativeformycoplasma,bacteria,yeast,andfungi.MEFareguaranteedtofurtherexpandfor5populationdoublingsundertheconditionsprovidedbyScienCellResearchLaboratories.

RecommendedMedium

ItisrecommendedtouseDMEM(Cat.#09221)supplementedwith10%fetalbovineserum(FBS,Cat.#0010,0025,0500)forculturingMEFinvitro.

Specifications:

美国Sciencell 研究实验室（ScienCell）是一家生物技术公司，其宗旨是研究和细胞和细胞发育相关的实验和治疗的产品。该ScienCell技术提供了高质量的正常人体和动物的各种细胞，细胞培养基和试剂，中补充，细胞源性核糖核酸，基因和蛋白质的研究团体。在ScienCell科学家还在研究和开发细胞治疗策略，将大大改善通过实现细胞的能力，打击贩运退行性疾病的生活质量。我们的总部设在加利福尼亚州圣迭戈，并于1999年设立。