MouseEmbryonicFibroblasts(MEF)areusedtosupportthegrowthofundifferentiatedmouseorhumanESandiPScells.TheyprovidebothasubstratefortheEScellstogrowonandsecretemanyfactorsnecessaryforEScellstomaintainpluripotency.MEFcellsareisolatedfrommouseembryosandareusedattheirearlypassages.Thesecellshavebeenmitomycintreatedtopreventfurthercelldivisionpriortocryopreservation.
MEFcellsfromSciencellResearchLaboratoriesareisolatedfromE14mouseembryos.ThesecellsarecryopreservedatP3aftermitomycinCtreatmentanddeliveredfrozen.Eachvialcontains1x10^6cellsin1mlvolume.MEF-mtarecharacterizedbyimmunofluorescentimagingusingantibodiestofibronectin.MEF-mtarenegativeformycoplasma,bacteria,yeastandfungi.
RecommendedMedium
ItisrecommendedtouseDMEM(Cat.#09221)supplementedwith10%fetalbovineserum(FBS,Cat.#0010,0025,0500)forculturingMEF-mtinvitro.
Specifications:
| CatalogNo. | M7540-mt |
| CountryofManufacture | UnitedStates |
| ProductCode | MEF-mt |
| Size/Quantity | 1x10^6cells/vial |
| ProductUse | MEFareusedasafeederlayerinmouseandhumanpluripotentstemcellculture.Theyareforresearchuseonly.Theyarenotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures. |
| Storage | Uponreceiving,directlyandimmediatelytransferthecellsfromdryicetoliquidnitrogenandkeepthecellsinliquidnitrogenuntiltheyareneededforexperiments. |
| ShippingInfo | Dryice. |
| References | [1]BRADLEY,A.(1987).Productionandanalysisofchimaeras.InTeratocarcinomasandEmbryonicStemCells:APracticalApproach,(ed.E.J.Robertson),pp.113-151.Oxford:IRLPress.[2]Nagyetal.(2006)PreparingMouseEmbryoFibroblastsColdSpringHarborProtocols.2006:pdb.prot4398. |
美国Sciencell 研究实验室（ScienCell）是一家生物技术公司，其宗旨是研究和细胞和细胞发育相关的实验和治疗的产品。该ScienCell技术提供了高质量的正常人体和动物的各种细胞，细胞培养基和试剂，中补充，细胞源性核糖核酸，基因和蛋白质的研究团体。在ScienCell科学家还在研究和开发细胞治疗策略，将大大改善通过实现细胞的能力，打击贩运退行性疾病的生活质量。我们的总部设在加利福尼亚州圣迭戈，并于1999年设立。
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