Multipotentmesenchymalstromalcells,alsoknownasmesenchymalstemcells(MSC),areawell-characterizedpopulationofadultstemcells.Theyhavethepotentialtodevelopintomaturecellsthatproducefat,cartilage,bone,tendons,andmuscle[1].Theseproperties,incombinationwiththeirdevelopmentalplasticity,havegeneratedtremendousinterestbecauseofthepotentialuseofMSCinregenerativemedicinetoreplacedamagedtissues.MSCculturedwithoutseruminthepresenceoftransformationgrowthfactorswilldifferentiateintochondrocytes.Incontrast,MSCculturedinserumwithascorbicacidanddexamethasonewilldifferentiateintoosteoblasts.MSChavethecapABIlityforrenewalanddifferentiationintovariouslineagesofmesenchymaltissues.MSCderivedfromtheamnionandchorionexpressCD271[2],possesscharacteristicsofcardiomyocytes[3],andhavevariousantiangiogenicandanti-inflammatoryproteins.
HAMSCfromSciencellResearchLaboratoriesareisolatedfromhumanamnioticmembrane.HAMSCarecryopreservedatpassageoneanddeliveredfrozen.Eachvialcontains>5x10^5cellsin1mlvolume.HAMSCarecharacterizedbyimmunofluorescencewithantibodiesspecifictoCD73,CD90,CD105andlipidstainingafterdifferentiation.HAMSCarenegativeforHIV-1,HBV,HCV,mycoplasma,bacteria,yeastandfungi.HAMSCareguaranteedtofurtherexpandfor15populationdoublingsundertheconditionsprovidedbyScienCellResearchLaboratories.
RecommendedMedium
ItisrecommendedtouseMesenchymalStemCellMedium(MSCM,Cat.#7501)forculturingHAMSCinvitro.
CatalogNo. | 7140 |
CountryofManufacture | UnitedStates |
ProductCode | HAMSC |
Size/Quantity | 5x10^5cells/vial |
ProductUse | HAMSCareforresearchuseonly.Itisnotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures. |
Storage | Directlyandimmediatelytransfercellsfromdryicetoliquidnitrogenuponreceivingandkeepthecellsinliquidnitrogenuntilcellcultureisneededforexperiments. |
ShippingInfo | Dryice. |
References | [1].Barry,F.P.,andJ.M.Murphy.Mesenchymalstemcells:clinicalapplicationsandBIOLOGicalcharacterization.2004.IntJBiochemCellBiol.36(4):568-84.[2].Soncini,M.,Vertua,E.,Gibelli,L.,Zorzi,F.,Denegri,M.,Albertini,A.,Wengler,G.andParolini,O.(2007)Isolationandcharacterizationofmesenchymalcellsfromhumanfetalmembranes.J.TissueEng.&Reg.Med.1(4):296-305.[3].Zhao,P.,Ise,H.,Hongo,M.,Ota,M.,Konishi,I.andNikaido,T.(2005)Humanamnioticmesenchymalcellshavesomecharacteristicsofcardiomyocytes.ExperimentalTransplantation79:528-535. |
美国Sciencell 研究实验室(ScienCell)是一家生物技术公司,其宗旨是研究和细胞和细胞发育相关的实验和治疗的产品。该ScienCell技术提供了高质量的正常人体和动物的各种细胞,细胞培养基和试剂,中补充,细胞源性核糖核酸,基因和蛋白质的研究团体。在ScienCell科学家还在研究和开发细胞治疗策略,将大大改善通过实现细胞的能力,打击贩运退行性疾病的生活质量。我们的总部设在加利福尼亚州圣迭戈,并于1999年设立。