EachprimersetincludedinHMSC-C-qPCRkitarriveslyophilizedina2mLvial.Allprimersaredesignedandtestedunderthesameparameters:(i)anoptimalannealingtemperatureof65°C(with2mMMg2+,andnoDMSO);(ii)recognitionofallknowntargetgenetranscriptvariants;and(iii)specificamplificationofonlyoneamplicon.
Humanmesenchymalstemcells(MSCs)areapopulationofmultipotentcellsthatcanbedifferentiatedintomultiplelineage-specificcells,whichcanformbone,fat,cartilage,muscleandtendon.Theprocessofcartilageformationfromcondensedmesenchymetissueisknownaschondrogenesis.SciencellhascreatedaconvenientqPCRkitfortheassessmentofhumanmesenchymalstemcellchondrogenesis.SOX9andCOL2A1qPCRprimersincludedinthekitallowforthedetectionandquantificationofearly-to-mid-andlate-stageofhumanMSCchondrogenesis,respectively1.
Note:allgenenamesfollowtheirofficialsymbolsbytheHumanGenomeOrganizationGeneNomenclatureCommittee(HGNC).
FourqPCRcontrolsareincludedinthiskittoverifysuccessfulreversetranscriptionofmessengerRNA(mRNA)tocomplementaryDNA(CDNA),revealthepresenceofgenomicDNA(gDNA)contaminationincDNAsamples,anddetectqPCRinhibitorcontamination.GoodqualitycDNAisacriticalcomponentforsuccessfulgeneexpressionanalysis.
EachprimersetincludedinHMSC-C-qPCRkitarriveslyophilizedina2mLvial.Allprimersaredesignedandtestedunderthesameparameters:(i)anoptimalannealingtemperatureof65°C(with2mMMg2+,andnoDMSO);(ii)recognitionofallknowntargetgenetranscriptvariants;and(iii)specificamplificationofonlyoneamplicon.EachprimersethasbeenvalidatedbyqPCRbymeltcurveanalysisandgelelectrophoresis.
CatalogNo. | 8328 |
CountryofManufacture | UnitedStates |
ProductCode | HMSC-C-qPCR |
Size/Quantity | 50reactions |
ProductUse | HMSC-CPCRkitisforresearchuseonly.Notforuseinanimals,humans,ordiagnosticprocedures. |
Storage | Storeat-20°Cuponreceipt.Avoidrepeatedfreezethawcyclesbymakingsixaliquotsat110μLeach. |
ShippingInfo | Dryice. |
References | [1]Sekiya,I.,Vuoristo,J.T.,Larson,B.L.,&Prockop,D.J.(2002).Invitrocartilageformationbyhumanadultstemcellsfrombonemarrowstromadefinesthesequenceofcellularandmoleculareventsduringchondrogenesis.PNAS,99(7),4397-4402. |
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