ThisassaykitisusedtoevaluatethecollagenIcelladhesioninvitro.Itisforresearchuseonly.Notforuseinanimals,humans,ordiagnosticprocedures.
Celladhesionplaysanimportantroleincellularcommunicationandregulation,andisoffundamentalimportanceinthedevelopmentandmaintenanceoftissues.CollagenI,amajorstructuralcomponentofextracellularmatrices,isanexcellentsubstrateforthecultureofmanydifferentcelltypes.The
Sciencell"CollagenICellAdhesionAssayisdesignedfortherapid,quantitativeandreliablemeasurementofcelladhesiontocollagenI.Thekitincludesa48-wellplatepre-coatedwithcollagenI,aswellasBSA(bovineserumalbumin)asnegativecontrols(seeplateformatinFigure1).Cellsareculturedinthepre-coatedwellsforadesiredperiodoftime,thenunboundedcellsarewashedaway,andtheadheredcellsarefixedandstained,followedbyanextractionstepwhichleadstodyeelutionfromstainedcellsintosupernatant.Thuscelladhesioncanbequantifiedusingacolorimetric
ELISAplatereaderat595nm.
Specifications:
CatalogNo. | 8008 |
CountryofManufacture | UnitedStates |
ProductCode | Col |
Size/Quantity | 48tests |
ProductUse | ThisassaykitisusedtoevaluatethecollagenIcelladhesioninvitro.Itisforresearchuseonly.Notforuseinanimals,humans,ordiagnosticprocedures. |
Storage | Medium4°C |
ShippingInfo | Gelice. |
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