Thisassaykitisusedfordetectionofapoptosis(programmedcelldeath)inindividualcellsbasedontheterminaldeoxynucleotidyltransferase(TdT)-mediateddUTP-biotinnick-end-labeling(TUNEL)technology.
The
SciencellColorimetricTUNELApoptosisAssayisusedfordetectionofapoptosis(programmedcelldeath)inindividualcellsbasedontheterminaldeoxynucleotidyltransferase(TdT)-mediateddUTP-biotinnick-end-labeling(TUNEL)technology.Inbrief,the3"-OHendoftheDNAstrandbreaksinapoptoticcellsislabeledwithbiotinylatednucleotidesusingtheenzymeTdT.Streptavidinconjugatedhorser
ADIshperoxidase(HRP)isthenboundtothebiotinylatednucleotides,andvisualizedusingtheperoxidasesubstrate,3,3"-diaminobenzidine(DAB).Thenucleiofapoptoticcellsshouldbeobserveddarkbrownunderlightmicroscope.
Specifications:
CatalogNo. | 8088 |
CountryofManufacture | UnitedStates |
ProductCode | TUNEL |
Size/Quantity | 50tests |
ProductUse | ThisassaykitisusedtoevaluateLDHactivityinvitro.Itisforresearchuseonly.Notforuseinanimals,humans,ordiagnosticprocedures. |
Storage | -20°C |
ShippingInfo | Dryice. |
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