Thisassaykitisusedfordetectionofapoptosis(programmedcelldeath)inindividualcellsbasedontheterminaldeoxynucleotidyltransferase(TdT)-mediateddUTP-biotinnick-end-labeling(TUNEL)technology.
The
SciencellColorimetricTUNELApoptosisAssayisusedfordetectionofapoptosis(programmedcelldeath)inindividualcellsbasedontheterminaldeoxynucleotidyltransferase(TdT)-mediateddUTP-biotinnick-end-labeling(TUNEL)technology.Inbrief,the3"-OHendoftheDNAstrandbreaksinapoptoticcellsislabeledwithbiotinylatednucleotidesusingtheenzymeTdT.Streptavidinconjugatedhorser
ADIshperoxidase(HRP)isthenboundtothebiotinylatednucleotides,andvisualizedusingtheperoxidasesubstrate,3,3"-diaminobenzidine(DAB).Thenucleiofapoptoticcellsshouldbeobserveddarkbrownunderlightmicroscope.
Specifications:
| CatalogNo. | 8088 |
| CountryofManufacture | UnitedStates |
| ProductCode | TUNEL |
| Size/Quantity | 50tests |
| ProductUse | ThisassaykitisusedtoevaluateLDHactivityinvitro.Itisforresearchuseonly.Notforuseinanimals,humans,ordiagnosticprocedures. |
| Storage | -20°C |
| ShippingInfo | Dryice. |
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