Thisassaykitisusedtoevaluatetubeformationinvitro.Itisforresearchuseonly.Notforuseinanimals,humans,ordiagnosticprocedures.
Angiogenesis,ortheformationofnewbloodvessels,playsanimportantroleinmanyphysiological(e.g.woundhealing)andpathological(e.g.tumorgrowthandinflammationdisease)processes.Asacomplexmulti-stepprocess,angiogenesisinvolvesdegradationofbasementmembrane,proliferationandmigrationofendothelialcellsintoextracellularmatrix(ECM),followedbyalignmentandreorganizationofendothelialcellsintothree-dimensionaltubularstructureandformationofnewbasementmembrane.Specifically,thecriticalstepoftheformationofthree-dimensionaltubularstructurebyendothelialcellsistheobjectiveofmanyinvivoandinvitroangiogenesisassaystoscreenefficacyofbothpro-andantiangiogenicagents.The
SciencellInVitroTubeFormationAssayprovidesaconvenientsystemtomeasuretheformationofthree-dimensionaltubularstructurebyenodothelialcellswhenculturedonanECMgelofbasementmembraneextract.TheECMgel,whichispreparedfromEngelbrethHolm-Swarm(EHS)sarcomaproducedinmice,consistsoflaminin,collagenIV,heparansulfateproteoglycan,entactinandotherminorcomponentsoptimizedfortubeformation.Withthissystem,tubeformationcanbeachievedwithin6-18hours,withtheearliestsignsoftubularstructureshownupafter3-4hours.TheAssayalsoprovidesacolorimetriccellstainingreagentforbetterexamination.
Specifications:
CatalogNo. | 8158 |
CountryofManufacture | UnitedStates |
ProductCode | IVTFA |
Size/Quantity | 50tests |
ProductUse | Thisassaykitisusedtoevaluatetubeformationinvitro.Itisforresearchuseonly.Notforuseinanimals,humans,ordiagnosticprocedures. |
Storage | -20°C |
ShippingInfo | Dryice. |
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