Thisassaykitisusedtoevaluatecaspase-3activityinvitro.Itisforresearchuseonly.Notforuseinanimals,humans,ordiagnosticprocedures.
Caspase-3isamemberofcaspasesthatplaysakeyroleinmediatingapoptosis,orprogrammedcelldeath.Uponactivation,itcleavesavarietyofcellularproteins,causingmorphologicalandfunctionalchangestocellsundergoingapoptosis.The
SciencellCaspase-3Assayprovidesaquickandconvenientmethodtomeasurecaspase-3activity.Thecolorimetricassayisbasedonthespectrophotometricdetectionofthechromophorep-nitroanilide(pNA)afteritscleavagebycaspases-3fromthelabeledsubstrateacetyl-Asp-glu-Val-Aspp-nitroanilide(Ac-DEVD-pNA).TheconcentrationofpNAismeasuredbyabsorbanceat405nm.Thecaspase-3activitycanbecalculatedasμmolofpNAreleasedperminutepermilliliterofcelllysate.
Specifications:
| CatalogNo. | 8228 |
| CountryofManufacture | UnitedStates |
| ProductCode | CAS |
| Size/Quantity | 100tests |
| ProductUse | THESEPRODUCTSAREFORRESEARCHUSEONLY.Notapprovedforhumanorveterinaryuse,forapplicationtohumansoranimals,orforuseinclinicalorinvitroprocedures. |
| Storage | -20°C |
| ShippingInfo | Dryice. |
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