TheMDHAssaykitmeasurestheMDHactivityindifferenttypesofsamples,includingtissueandcelllysate.Thisproductisforresearchpurposesonlyandisnotforuseinanimals,humans,ordiagnosticprocedures.
Malatedehydrogenase(MDH2)catalyzesthelastreactionoftheTCAorKrebscycle,convertingmalateandNAD+tooxaloacetateandNADHinthemitochondria.Inreversingthereaction,MDHisalsoinvolvedingluconeogenesis,allowingoxaloacetate/malatetoleavethemitochondria.Onceinthecytosol,themalateisoxidizedbacktooxaloacetatebycytosolicMDHorMDH1,whichisfollowedbyconversionofoxaloacetatetophosphoenolpyruvatebyphosphoenolpyruvatecarboxykinase(PEPCK).Theinterconversionbetweenmalateandoxaloacetatealsoconstitutestheessentialstepsofthemalate-aspartateshuttle.Thiscolorimetricassayisbasedonmalatedehydrogenase-catalyzedoxidationofmalate,wheretheresultingNADHcanthenconvertanearlycolorlessprobetoacoloredproduct;theintensityofthecoloredproductisproportionaltotheamountofMDHinthesample,exhibitingmaximumabsorbanceat440nm.
Specifications:
CatalogNo. | 8638 |
CountryofManufacture | UnitedStates |
ProductCode | MDH |
Size/Quantity | 100Tests |
ProductUse | TheMDHAssaykitmeasurestheMDHactivityindifferenttypesofsamples,includingtissueandcelllysate.Thisproductisforresearchpurposesonlyandisnotforuseinanimals,humans,ordiagnosticprocedures. |
Storage | Storein-20°C |
ShippingInfo | Shippedondryice. |
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