TheABIlityofhumanembryonicandinducedpluripotentstemcellstodifferentiateintoallthreegermlayershasenormouspotentialforbasichumandevelopmentalresearchandregenerativemedicine.SciencellhascreatedaconvenientqPCRkitfortheassessmentofhumanpluripotentstemcelldifferentiation.NEUROD1,ACTA2andAFPqPCRprimersincludedinthekitallowforthedetection andquantificationofectoderm,mesodermandendoderm,respectively1.
Note:allgenenamesfollowtheirofficialsymbolsbytheHumanGenomeOrganizationGeneNomenclatureCommittee(HGNC).
FourqPCRcontrolsareincludedinthiskittoverifysuccessfulreversetranscriptionofmessengerRNA(mRNA)tocomplementaryDNA(cDNA),revealthepresenceofgenomicDNA(gDNA)contaminationincDNAsamples,anddetectqPCRinhibitorcontamination.GoodqualitycDNAisacriticalcomponentforsuccessfulgeneexpressionanalysis.
EachprimersetincludedinHGL-qPCRkitarriveslyophilizedina2mLvial.Allprimersaredesignedandtestedunderthesameparameters:(i)anoptimalannealingtemperatureof65°C(with2mMMg2+,andnoDMSO);(ii)recognitionofallknowntargetgenetranscriptvariants;and(iii)specificamplificationofonlyoneamplicon.EachprimersethasbeenvalidatedbyqPCRbymeltcurveanalysisandgelelectrophoresis.
CatalogNo. | 0833 |
CountryofManufacture | UnitedStates |
ProductCode | HGL-qPCR |
Size/Quantity | 50reactions |
ProductUse | HGLPCRkitisforresearchuseonly.Itisnotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures. |
Storage | Storein-20°Cuponreceipt.Avoidrepeatedfreezethawcyclesbymakingfivealiquotsat170μLeach. |
ShippingInfo | Dryice. |
References | [1]Adewumi,O.etal.(2007)CharacterizationofhumanembryonicstemcelllinesbytheInternationalStemCellInitiative.NatBiotechnol.25:803-816. |
美国Sciencell 研究实验室(ScienCell)是一家生物技术公司,其宗旨是研究和细胞和细胞发育相关的实验和治疗的产品。该ScienCell技术提供了高质量的正常人体和动物的各种细胞,细胞培养基和试剂,中补充,细胞源性核糖核酸,基因和蛋白质的研究团体。在ScienCell科学家还在研究和开发细胞治疗策略,将大大改善通过实现细胞的能力,打击贩运退行性疾病的生活质量。我们的总部设在加利福尼亚州圣迭戈,并于1999年设立。